Hotspot

rps15-ycf1

SSC · 123085-123511 · 427 bp

Back to Verbascum

Pi 0.00782

MarkerSeek score 42.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 427
variable_sites: 7
pi: 0.00782
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 42.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGAGTCATTCTTCCCATGCA	GAACCCATTCGTCTGTCTGG	50.0	55.0	58.0	58.3	1278-1315 bp	1.0	80.9
rps15-ycf1_p2	CCCGAGTCATTCTTCCCATG	GAACCCATTCGTCTGTCTGG	55.0	55.0	58.0	58.3	1280-1317 bp	1.0	80.8
rps15-ycf1_p3	TCCCATGCATTGCTGAAAGT	GAACCCATTCGTCTGTCTGG	45.0	55.0	58.1	58.3	1267-1304 bp	1.0	80.6
rps15-ycf1_p4	AACACCATGCATTGATGGGT	GAACCCATTCGTCTGTCTGG	45.0	55.0	58.1	58.3	1364-1401 bp	1.0	80.5
rps15-ycf1_p5	GACCCCCTGTTTTCTTCGTT	GAACCCATTCGTCTGTCTGG	50.0	55.0	58.0	58.3	980-1017 bp	0.8	73.5