Hotspot

rpl32-trnL-UAG

SSC · 113084-113967 · 884 bp

Back to Verbascum

Pi 0.00885
MarkerSeek score 69.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
884
variable_sites
14
pi
0.00885
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GTATTGGGCAGCGCTAAAAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.5 57.9 996-1029 bp 1.0 79.2
rpl32-trnL-UAG_p2 AAAGAAGGGGTATTGGGCAG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.5 57.9 1005-1038 bp 1.0 78.7
rpl32-trnL-UAG_p3 GTATTGGGCAGCGCTAAAAG AGCAGCGTGTCTACCAATTT 50.0 45.0 57.5 57.8 989-1022 bp 1.0 78.7
rpl32-trnL-UAG_p4 GTATTGGGCAGCGCTAAAAG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.5 57.8 995-1028 bp 1.0 78.6
rpl32-trnL-UAG_p5 GTATTGGGCAGCGCTAAAAG CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.5 57.8 994-1027 bp 1.0 78.6