Hotspot

ycf4-cemA

LSC · 61346-62005 · 660 bp

Back to Verbascum

Pi 0.00848

MarkerSeek score 57.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 13
pi: 0.00848
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTCCCTTGACCCGTACTGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	835-844 bp	1.0	80.2
ycf4-cemA_p2	TTCCCTTGACCCGTACTGAT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	834-843 bp	1.0	80.2
ycf4-cemA_p3	AGCTATTCCCTTGACCCGTA	GAGAAATCCACCAGGGCAAA	50.0	50.0	57.8	58.1	839-848 bp	1.0	79.3
ycf4-cemA_p4	TATTTCTTGCGCGTACCGAT	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	766-775 bp	1.0	79.0
ycf4-cemA_p5	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	790-799 bp	1.0	78.9