Hotspot

rps2-rpoC2

LSC · 16436-16655 · 220 bp

Back to Verbascum

Pi 0.0162

MarkerSeek score 66.20

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 7
pi: 0.0162
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCCTCCAGCATTTCTTCCAA	TTCGAGGGGGAAATAAGAGA	50.0	45.0	58.5	55.2	380-384 bp	1.0	56.8
rps2-rpoC2_p2	AAATGAACTCCTGCCTCCAG	TTCGAGGGGGAAATAAGAGA	50.0	45.0	57.5	55.2	392-396 bp	1.0	56.3
rps2-rpoC2_p3	TTGCGGAGATATAAGGTGCC	TTCGAGGGGGAAATAAGAGA	50.0	45.0	57.5	55.2	445-449 bp	1.0	55.7
rps2-rpoC2_p4	TGCGGAGATATAAGGTGCCA	TTCGAGGGGGAAATAAGAGA	50.0	45.0	58.6	55.2	444-448 bp	1.0	55.4
rps2-rpoC2_p5	CCATGGCCAAAATGAACTCC	TTCGAGGGGGAAATAAGAGA	50.0	45.0	57.3	55.2	401-405 bp	1.0	54.9