Hotspot

ycf1

SSC · 108704-114100 · 5397 bp

Back to Veratrum

Pi 0.017

MarkerSeek score 52.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5397
variable_sites: 220
pi: 0.017
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGACCATTTCGCATAACA	TCGTACCACTCAGGCAATTG	45.0	50.0	57.9	57.9	1048-1075 bp	1.0	83.3
ycf1_p2	AACAGAAGAGATCCGGGTGA	TCGTACCACTCAGGCAATTG	50.0	50.0	58.1	57.9	1536-1563 bp	1.0	83.0
ycf1_p3	ACAGAAGAGATCCGGGTGAA	TCGTACCACTCAGGCAATTG	50.0	50.0	58.1	57.9	1535-1562 bp	1.0	83.0
ycf1_p4	AAGAGATCCGGGTGAATGGA	TCGTACCACTCAGGCAATTG	50.0	50.0	58.1	57.9	1531-1558 bp	1.0	82.6
ycf1_p5	AGAGATCCGGGTGAATGGAA	TCGTACCACTCAGGCAATTG	50.0	50.0	58.1	57.9	1530-1557 bp	1.0	82.6