Hotspot

ndhF

SSC · 125094-127292 · 2199 bp

Back to Veratrum

Pi 0.0173

MarkerSeek score 48.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2199
variable_sites: 88
pi: 0.0173
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACCGCCATTCAAAAAGGAGT	AACAGAAGAGATCCGGGTGA	45.0	50.0	58.0	58.1	2741-2871 bp	0.9	82.6
ndhF_p2	ACCGCCATTCAAAAAGGAGT	ACAGAAGAGATCCGGGTGAA	45.0	50.0	58.0	58.1	2740-2870 bp	0.9	82.6
ndhF_p3	ACCGCCATTCAAAAAGGAGT	AAACACGAACAAGGGGGATC	45.0	50.0	58.0	58.1	2821-2951 bp	0.9	82.1
ndhF_p4	ACCGCCATTCAAAAAGGAGT	AAGAGATCCGGGTGAATGGA	45.0	50.0	58.0	58.1	2736-2866 bp	0.9	82.1
ndhF_p5	ACCGCCATTCAAAAAGGAGT	AGAGATCCGGGTGAATGGAA	45.0	50.0	58.0	58.1	2735-2865 bp	0.9	82.1