Hotspot

rpl32-ndhF

SSC · 124571-125093 · 523 bp

Back to Veratrum

Pi 0.026
MarkerSeek score 57.60
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
523
variable_sites
33
pi
0.026
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.85
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 CCCTTCTTCTTCCAAATATTTTTACG TGCATATTGATATGTATGTTCCA 34.6 30.4 57.1 53.7 521-620 bp 1.0 48.7
rpl32-ndhF_p2 TCCCTTCTTCTTCCAAATATT TGCATATTGATATGTATGTTCCA 33.3 30.4 52.1 53.7 522-621 bp 1.0 48.7
rpl32-ndhF_p3 TCCCTTCTTCTTCCAAATATTT TGCATATTGATATGTATGTTCCA 31.8 30.4 53.0 53.7 522-621 bp 1.0 48.7
rpl32-ndhF_p4 CCCTTCTTCTTCCAAATATTTTTACG TCAATGCATATTGATATGTATGTTCCA 34.6 29.6 57.1 57.5 525-624 bp 1.0 48.7
rpl32-ndhF_p5 TCCCTTCTTCTTCCAAATATT TCAATGCATATTGATATGTATGTTCCA 33.3 29.6 52.1 57.5 526-625 bp 1.0 48.7