Hotspot

ndhA-ndhI

SSC · 118203-118284 · 82 bp

Back to Veratrum

Pi 0.0336

MarkerSeek score 59.00

Primer available yes

Label ndhA-ndhI

Feature Metadata

label_name: ndhA-ndhI
feature_type: igs
region: SSC
length_bp: 82
variable_sites: 6
pi: 0.0336
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA-ndhI_p1	GGATGAGAATGGACCAGTTGT	CTTGCGGCTCGTATTGTTTG	47.6	50.0	57.6	58.1	218-245 bp	1.0	74.2
ndhA-ndhI_p2	ACCTAGGATGAGAATGGACCA	CTTGCGGCTCGTATTGTTTG	47.6	50.0	57.6	58.1	223-250 bp	1.0	73.6
ndhA-ndhI_p3	GGATGAGAATGGACCAGTTGT	GCGGCTCGTATTGTTTGTTG	47.6	50.0	57.6	58.4	215-242 bp	1.0	72.0
ndhA-ndhI_p4	AGGATGAGAATGGACCAGTTG	CTTGCGGCTCGTATTGTTTG	47.6	50.0	57.4	58.1	219-246 bp	1.0	71.9
ndhA-ndhI_p5	ACCTAGGATGAGAATGGACCA	GCGGCTCGTATTGTTTGTTG	47.6	50.0	57.6	58.4	220-247 bp	1.0	71.4