Hotspot

ycf1-rps15

SSC · 114101-114484 · 384 bp

Back to Veratrum

Pi 0.0346

MarkerSeek score 54.70

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 384
variable_sites: 28
pi: 0.0346
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	GGAGAGATACGCCCGGATAT	AACACCATGCATTGATGGGT	55.0	45.0	58.2	58.1	1427-1452 bp	1.0	82.5
ycf1-rps15_p2	TTGCTTCTTTGGCCCGATTA	GACAAATAAGCCAGCAAGCG	45.0	50.0	57.8	58.1	716-724 bp	0.7	69.0
ycf1-rps15_p3	TTGCTTCTTTGGCCCGATTA	AACACCATGCATTGATGGGT	45.0	45.0	57.8	58.1	961-969 bp	0.7	68.9
ycf1-rps15_p4	TTGCTTCTTTGGCCCGATTA	TGAGATCCTTTCTTGTGGCG	45.0	50.0	57.8	57.9	917-925 bp	0.7	68.8
ycf1-rps15_p5	TTGCTTCTTTGGCCCGATTA	GATCCTTTCTTGTGGCGAGT	45.0	50.0	57.8	57.9	914 bp	0.1	41.5