Hotspot

rpl22-rps19

LSC · 82972-83055 · 84 bp

Back to Veratrum

Pi 0.0317
MarkerSeek score 56.50
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
84
variable_sites
6
pi
0.0317
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TACTTCTGTACTCGAACACGA CAAGACATGCAAGAAACGAT 42.9 40.0 56.2 54.6 154-161 bp 1.0 47.0
rpl22-rps19_p2 TTACTTCTGTACTCGAACACGA CAAGACATGCAAGAAACGAT 40.9 40.0 56.9 54.6 155-162 bp 1.0 46.9
rpl22-rps19_p3 ACTTCTGTACTCGAACACGA CAAGACATGCAAGAAACGAT 45.0 40.0 56.3 54.6 153-160 bp 1.0 46.5
rpl22-rps19_p4 ACTTCTGTACTCGAACACGA CAAGACATGCAAGAAACGATA 45.0 38.1 56.3 54.6 153-160 bp 1.0 46.5
rpl22-rps19_p5 ACTTCTGTACTCGAACACGA CAAGACATGCAAGAAACGATAA 45.0 36.4 56.3 55.4 153-160 bp 1.0 46.5