Hotspot

petD-rpoA

LSC · 76931-77060 · 130 bp

Back to Veratrum

Pi 0.0574

MarkerSeek score 58.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 130
variable_sites: 20
pi: 0.0574
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCCCACTTGGATATTGAGCA	TTAGAGACGCCTCCATTTGC	50.0	50.0	57.9	58.0	1599-1677 bp	1.0	82.5
petD-rpoA_p2	CTCGAGCCGGATGATGAAAA	TTAGAGACGCCTCCATTTGC	50.0	50.0	58.1	58.0	1412-1497 bp	1.0	82.3
petD-rpoA_p3	GCCCACTTGGATATTGAGCA	TGCTGTATCCATGCCAGTTC	50.0	50.0	57.9	58.0	1382-1460 bp	1.0	82.3
petD-rpoA_p4	CTCGAGCCGGATGATGAAAA	TGCTGTATCCATGCCAGTTC	50.0	50.0	58.1	58.0	1195-1280 bp	1.0	82.1
petD-rpoA_p5	GCCCACTTGGATATTGAGCA	TTTGCGTAAGGGGTCCTAGA	50.0	50.0	57.9	58.1	1584-1662 bp	0.7	69.1