Hotspot

psbT-psbN

LSC · 73419-73482 · 64 bp

Back to Veratrum

Pi 0.0316

MarkerSeek score 66.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 7
pi: 0.0316
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TTTTCGAGAACCGCCTAAGG	AAGCTTTACTGGGTACGCTT	50.0	45.0	57.9	57.1	186 bp	1.0	75.5
psbT-psbN_p2	TTTTCGAGAACCGCCTAAGG	AGCTTTACTGGGTACGCTTT	50.0	45.0	57.9	57.1	185 bp	1.0	75.5
psbT-psbN_p3	TTTTCGAGAACCGCCTAAGG	TGTAAGCTTTACTGGGTACGC	50.0	47.6	57.9	58.0	189 bp	1.0	74.0
psbT-psbN_p4	TTTTCGAGAACCGCCTAAGG	AAGCTTTACTGGGTACGCTTT	50.0	42.9	57.9	57.9	186 bp	1.0	73.2
psbT-psbN_p5	TTTTCGAGAACCGCCTAAGG	GTAAGCTTTACTGGGTACGCT	50.0	47.6	57.9	57.8	188 bp	1.0	72.3