Hotspot

rpoB-trnC-GCA

LSC · 26374-27308 · 935 bp

Back to Veratrum

Pi 0.0214

MarkerSeek score 53.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 935
variable_sites: 47
pi: 0.0214
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCCGCCTACACAAGCAAATT	AACTTGTTTAGCAGGCGACA	45.0	45.0	58.1	58.0	1723-1727 bp	1.0	82.8
rpoB-trnC-GCA_p2	TCCGCCTACACAAGCAAATT	GATTTCTCGTCGGCCTATCC	45.0	55.0	58.1	57.9	2181-2185 bp	1.0	82.5
rpoB-trnC-GCA_p3	TCCGCCTACACAAGCAAATT	GGATTTCTCGTCGGCCTATC	45.0	55.0	58.1	57.9	2182-2186 bp	1.0	82.5
rpoB-trnC-GCA_p4	TCCGCCTACACAAGCAAATT	TTCAGCCCTTTCCACTCCTA	45.0	50.0	58.1	58.0	2207-2211 bp	0.8	74.3
rpoB-trnC-GCA_p5	TCCGCCTACACAAGCAAATT	AATGGCTCGAACCAATCCTC	45.0	50.0	58.1	58.0	1946 bp	0.1	42.9