Hotspot

psbI-trnS-GCU

LSC · 7570-7693 · 124 bp

Back to Veratrum

Pi 0.0595

MarkerSeek score 64.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 15
pi: 0.0595
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	200-221 bp	1.0	81.9
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	ATTCGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.2	224-245 bp	1.0	79.9
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	TAAAGCGGCGGATTGCTAAT	47.6	45.0	57.8	58.0	200-221 bp	1.0	79.5
psbI-trnS-GCU_p4	CGTAATCCTGGACGTGAAGA	ATGGCTGAGTGGACTAAAGC	50.0	50.0	57.1	57.6	214-235 bp	1.0	78.5
psbI-trnS-GCU_p5	CGTAATCCTGGACGTGAAGAA	ATTCGGAGAGATGGCTGAGT	47.6	50.0	57.8	58.2	224-245 bp	1.0	77.6