Hotspot

ycf1

IRb · 112243-113451 · 1209 bp

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Pi 0.00797
MarkerSeek score 63.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
1209
variable_sites
31
pi
0.00797
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGAGATGTCGTTTCTAGTCT TGGAATTACGAATGGGGTCG 40.0 50.0 52.9 57.7 1310-1485 bp 1.0 44.6
ycf1_p2 AGAGATGTCGTTTCTAGTCT AGGATAAGAGGATTGGCGGA 40.0 50.0 52.9 57.9 1363-1538 bp 1.0 44.4
ycf1_p3 AAGAGATGTCGTTTCTAGTCT AGGATAAGAGGATTGGCGGA 38.1 50.0 53.8 57.9 1364-1539 bp 1.0 44.4
ycf1_p4 GAGATGTCGTTTCTAGTCTATCTGT AGGATAAGAGGATTGGCGGA 40.0 50.0 57.7 57.9 1362-1537 bp 1.0 44.4
ycf1_p5 AGATGTCGTTTCTAGTCTATCTGT AGGATAAGAGGATTGGCGGA 37.5 50.0 56.7 57.9 1361-1536 bp 1.0 44.4