Hotspot

ndhF

SSC · 113330-115555 · 2226 bp

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Pi 0.00715
MarkerSeek score 44.10
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2226
variable_sites
65
pi
0.00715
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 TATCCGCCGAAGAAGATCCT TTTATTGGGGAACGCTCTGG 50.0 50.0 58.0 57.9 2820-2903 bp 0.923077 79.7
ndhF_p2 CAGGAACAAGAGGTATCCGC TTTATTGGGGAACGCTCTGG 55.0 50.0 58.1 57.9 2833-2916 bp 0.923077 79.3
ndhF_p3 GGTATCCGCCGAAGAAGATC TTTATTGGGGAACGCTCTGG 55.0 50.0 57.9 57.9 2822-2905 bp 0.923077 79.2
ndhF_p4 GTATCCGCCGAAGAAGATCC TTTATTGGGGAACGCTCTGG 55.0 50.0 57.9 57.9 2821-2904 bp 0.923077 79.2
ndhF_p5 TATCCGCCGAAGAAGATCCT GGCCAGCCTATGATTTCGAT 50.0 50.0 58.0 57.8 2829-2833 bp 0.153846 44.7