Hotspot

atpF

LSC · 12160-13538 · 1379 bp

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Pi 0.00772
MarkerSeek score 50.20
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1379
variable_sites
38
pi
0.00772
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 ATGTGCCTACAGTAAAAGGA AGTCTATCTAAAAGAGGAGATCA 40.0 34.8 54.0 53.5 1420-1433 bp 0.846154 37.7
atpF_p2 AATGTGCCTACAGTAAAAGGA AGTCTATCTAAAAGAGGAGATCA 38.1 34.8 54.8 53.5 1421-1434 bp 0.846154 37.7
atpF_p3 ATGTGCCTACAGTAAAAGGA AGTCTATCTAAAAGAGGAGATCAT 40.0 33.3 54.0 54.1 1420-1433 bp 0.846154 37.7
atpF_p4 AATGTGCCTACAGTAAAAGGA AGTCTATCTAAAAGAGGAGATCAT 38.1 33.3 54.8 54.1 1421-1434 bp 0.846154 37.7
atpF_p5 AATGTGCCTACAGTAAAAGG AGTCTATCTAAAAGAGGAGATCA 40.0 34.8 53.2 53.5 1421-1434 bp 0.846154 37.7