Hotspot

rps15-ycf1

SSC · 127204-131982 · 4779 bp

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Pi 0.0122
MarkerSeek score 51.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
4779
variable_sites
242
pi
0.0122
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TGGAAGACAAGACATGTATAC TGTATGAATCGCTATTGGTT 38.1 35.0 52.7 52.4 167 bp 1.0 45.7
rps15-ycf1_p2 TGGAAGACAAGACATGTATAC TTGTATGAATCGCTATTGGT 38.1 35.0 52.7 52.4 168 bp 1.0 45.7
rps15-ycf1_p3 TGGAAGACAAGACATGTATAC TGTATGAATCGCTATTGGTTT 38.1 33.3 52.7 53.3 167 bp 1.0 45.7
rps15-ycf1_p4 TGGAAGACAAGACATGTATAC TTGTATGAATCGCTATTGGTT 38.1 33.3 52.7 53.3 168 bp 1.0 45.7
rps15-ycf1_p5 TGGAAGACAAGACATGTATAC TTTGTATGAATCGCTATTGGT 38.1 33.3 52.7 53.3 169 bp 1.0 45.7