Hotspot

ndhG-ndhI

SSC · 122450-122835 · 386 bp

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Pi 0.00903
MarkerSeek score 61.80
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
386
variable_sites
18
pi
0.00903
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AATACCACTCCCAGACCTCC ACGACCGTCACGAATTGAAT 55.0 45.0 58.1 57.9 583-599 bp 1.0 83.2
ndhG-ndhI_p2 CCACTCCCAGACCTCCTAAT ACGACCGTCACGAATTGAAT 55.0 45.0 57.8 57.9 579-595 bp 1.0 82.7
ndhG-ndhI_p3 TACCACTCCCAGACCTCCTA ACGACCGTCACGAATTGAAT 55.0 45.0 58.3 57.9 581-597 bp 1.0 81.7
ndhG-ndhI_p4 ATTGGTCCAGGCAAATCCAT ACGACCGTCACGAATTGAAT 45.0 45.0 57.4 57.9 520-536 bp 1.0 80.1
ndhG-ndhI_p5 AATACCACTCCCAGACCTCC CGAACAGTCTTGAATTCGCC 55.0 50.0 58.1 57.5 506-522 bp 0.923077 76.6