Hotspot

psbB-psbT

LSC · 76458-76672 · 215 bp

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Pi 0.0283
MarkerSeek score 64.80
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
215
variable_sites
23
pi
0.0283
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 GGAGATCCAACTACAAGAAGACA GAACTTTAGGCGGTTCTCGA 43.5 50.0 57.6 57.6 321-337 bp 1.0 59.4
psbB-psbT_p2 TGGAGATCCAACTACAAGAAGAC GAACTTTAGGCGGTTCTCGA 43.5 50.0 57.6 57.6 322-338 bp 1.0 59.4
psbB-psbT_p3 GGAGATCCAACTACAAGAAGACA TGGAACTTTAGGCGGTTCTC 43.5 50.0 57.6 57.5 323-339 bp 1.0 58.5
psbB-psbT_p4 TGGAGATCCAACTACAAGAAGAC TGGAACTTTAGGCGGTTCTC 43.5 50.0 57.6 57.5 324-340 bp 1.0 58.5
psbB-psbT_p5 TCCAAAAACTTGGAGATCCA GAACTTTAGGCGGTTCTCGA 40.0 50.0 54.4 57.6 332-348 bp 1.0 58.0