Hotspot

petN-psbM

LSC · 30039-31365 · 1327 bp

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Pi 0.0163
MarkerSeek score 67.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1327
variable_sites
72
pi
0.0163
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACATTTTCCCTTTCGCTCGT TGTTCGTTCTAGTTCCTACTGC 45.0 45.5 58.1 57.8 971-1496 bp 0.923077 66.8
petN-psbM_p2 CCCTTTCGCTCGTAGTATGG TGTTCGTTCTAGTTCCTACTGC 55.0 45.5 57.9 57.8 964-1489 bp 0.923077 66.6
petN-psbM_p3 CCTTTCGCTCGTAGTATGGG TGTTCGTTCTAGTTCCTACTGC 55.0 45.5 57.9 57.8 963-1488 bp 0.923077 66.6
petN-psbM_p4 CTTTCGCTCGTAGTATGGGG TGTTCGTTCTAGTTCCTACTGC 55.0 45.5 57.9 57.8 962-1487 bp 0.923077 66.6
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT TGTTCGTTCTAGTTCCTACTGC 50.0 45.5 58.2 57.8 1009-1534 bp 0.923077 66.3