Hotspot

rpoB-trnC-GCA

LSC · 27872-29148 · 1277 bp

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Pi 0.0164
MarkerSeek score 65.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1277
variable_sites
69
pi
0.0164
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 ATTCCCGCATTTCCATCTCC CGCCAAGCAGAATAAGGGAT 50.0 50.0 58.0 58.0 1381-1511 bp 0.923077 84.5
rpoB-trnC-GCA_p2 ATTCCCGCATTTCCATCTCC ATCGTTTGTTGATCAGGCGA 50.0 45.0 58.0 57.9 1351-1481 bp 0.923077 84.0
rpoB-trnC-GCA_p3 ATTCCCGCATTTCCATCTCC GTTTGTTGATCAGGCGACAC 50.0 50.0 58.0 57.7 1348-1478 bp 0.923077 82.3
rpoB-trnC-GCA_p4 ATTCCCGCATTTCCATCTCC TACCCGCCAAGCAGAATAAG 50.0 50.0 58.0 57.7 1385-1515 bp 0.923077 82.1
rpoB-trnC-GCA_p5 ATTCCCGCATTTCCATCTCC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1310-1440 bp 0.923077 82.1