Hotspot

atpF-atpH

LSC · 13539-14000 · 462 bp

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Pi 0.0159
MarkerSeek score 68.80
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
462
variable_sites
27
pi
0.0159
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG TGGACTGGTTGTAGCATTAGC 50.0 47.6 57.9 58.0 579-594 bp 0.923077 74.6
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG GGAGGGCAAAATACGAGGTA 50.0 50.0 57.9 57.0 642-657 bp 0.923077 74.2
atpF-atpH_p3 TGGCCAGTGAACCAAAGAAA GGAGGGCAAAATACGAGGTA 45.0 50.0 57.8 57.0 618-633 bp 0.923077 74.2
atpF-atpH_p4 GCCAGTGAACCAAAGAAACG TGGACTGGTTGTAGCATTAGC 50.0 47.6 57.9 58.0 553-568 bp 0.846154 71.2
atpF-atpH_p5 GCCAGTGAACCAAAGAAACG GGAGGGCAAAATACGAGGTA 50.0 50.0 57.9 57.0 616-631 bp 0.846154 70.7