Hotspot

trnT-CGU-trnR-UCU

LSC · 10104-10277 · 174 bp

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Pi 0.0317
MarkerSeek score 65.50
Primer available yes
Label trnT-CGU-trnR-UCU

Feature Metadata

label_name
trnT-CGU-trnR-UCU
feature_type
igs
region
LSC
length_bp
174
variable_sites
21
pi
0.0317
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-CGU-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG TGGAAATCAATTGCGTCCAA 50.0 40.0 58.0 56.2 304-312 bp 0.923077 68.0
trnT-CGU-trnR-UCU_p2 CCTAGCCTTCCAAGCTAACG TGGAAATCAATTGCGTCCAA 55.0 40.0 58.1 56.2 307-315 bp 0.923077 67.7
trnT-CGU-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 257-265 bp 0.923077 66.8
trnT-CGU-trnR-UCU_p4 AGCCTTCCAAGCTAACGATG ATGGAAATCAATTGCGTCCA 50.0 40.0 58.0 56.0 305-313 bp 0.923077 66.1
trnT-CGU-trnR-UCU_p5 CCTAGCCTTCCAAGCTAACG ATGGAAATCAATTGCGTCCA 55.0 40.0 58.1 56.0 308-316 bp 0.923077 65.8