Hotspot

trnK-UUU-rps16

LSC · 4450-5023 · 574 bp

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Pi 0.0223
MarkerSeek score 76.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
574
variable_sites
45
pi
0.0223
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGCCGTACTTAAAAGCCGAG ATTTCAAAGAAAGCGGGGGT 50.0 45.0 58.0 58.0 664-692 bp 0.923077 85.8
trnK-UUU-rps16_p2 CTCTCCCGAAGATTTGGACG ATTTCAAAGAAAGCGGGGGT 55.0 45.0 58.1 58.0 719-747 bp 0.923077 84.9
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT ATTTCAAAGAAAGCGGGGGT 50.0 45.0 58.2 58.0 653-681 bp 0.923077 84.6
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT ATTTCAAAGAAAGCGGGGGT 50.0 45.0 58.2 58.0 652-680 bp 0.923077 84.6
trnK-UUU-rps16_p5 AGCCGTACTTAAAAGCCGAG CCAACCTACAGGAACCGTTC 50.0 55.0 58.0 58.2 689-717 bp 0.923077 84.0