Hotspot

trnH-GUG-psbA

LSC · 99-505 · 407 bp

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Pi 0.0688
MarkerSeek score 75.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
407
variable_sites
98
pi
0.0688
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CAATCCACTGCCTTGATCCA AGCTGCTATTGAAGTTCCATCT 50.0 40.9 57.9 57.5 457-508 bp 0.923077 67.9
trnH-GUG-psbA_p2 AATCCACTGCCTTGATCCAC AGCTGCTATTGAAGTTCCATCT 50.0 40.9 57.9 57.5 456-507 bp 0.923077 67.8
trnH-GUG-psbA_p3 ACAATCCACTGCCTTGATCC AGCTGCTATTGAAGTTCCATCT 50.0 40.9 57.9 57.5 458-509 bp 0.923077 67.8
trnH-GUG-psbA_p4 CGCATGGTGGATTCACAATC AGCTGCTATTGAAGTTCCATCT 50.0 40.9 57.5 57.5 472-523 bp 0.923077 65.1
trnH-GUG-psbA_p5 CAATCCACTGCCTTGATCCA GCTGCTATTGAAGTTCCATCT 50.0 42.9 57.9 56.0 456-507 bp 0.923077 64.1