Hotspot

ycf1

IRb · 115925-117040 · 1116 bp

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Pi 0.0364

MarkerSeek score 68.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1116
variable_sites: 39
pi: 0.0364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	TTGGTCATACAATCGCGGTT	55.0	45.0	58.0	57.9	1627-1680 bp	1.0	85.0
ycf1_p2	CCGGAACTTCTTCGTAGTGG	TTGGTCATACAATCGCGGTT	55.0	45.0	58.0	57.9	1626-1679 bp	1.0	85.0
ycf1_p3	TCCCGGAACTTCTTCGTAGT	TTGGTCATACAATCGCGGTT	50.0	45.0	58.1	57.9	1628-1681 bp	1.0	84.4
ycf1_p4	TCAAAGCTCCCCAAGTAGGA	TTGGTCATACAATCGCGGTT	50.0	45.0	58.0	57.9	1787-1840 bp	1.0	84.3
ycf1_p5	AGGATTATCCCGGACCTACG	TTGGTCATACAATCGCGGTT	55.0	45.0	58.1	57.9	1960-2013 bp	1.0	83.5