Hotspot

ndhF

SSC · 117113-119362 · 2250 bp

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Pi 0.0133

MarkerSeek score 50.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 30
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	AGAGCCGATGAATCAGAAACT	50.0	42.9	58.0	57.1	2610-2664 bp	1.0	69.0
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGAGCCGATGAATCAGAAACT	50.0	42.9	58.0	57.1	2596-2650 bp	1.0	68.8
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGAGCCGATGAATCAGAAACT	50.0	42.9	58.0	57.1	2588-2642 bp	1.0	68.8
ndhF_p4	GCAGAATACCGTCACCCATT	AGAGCCGATGAATCAGAAACT	50.0	42.9	58.0	57.1	2720-2774 bp	1.0	68.7
ndhF_p5	AGGGATCCACCGAAGAAGAT	AGAGCCGATGAATCAGAAACT	50.0	42.9	57.8	57.1	2585-2639 bp	1.0	67.7