Hotspot

rps15-ycf1

SSC · 131271-131639 · 369 bp

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Pi 0.0528

MarkerSeek score 71.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 369
variable_sites: 18
pi: 0.0528
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	ATGCCTAGAAATGCAGCGTT	50.0	45.0	58.0	58.2	1170-1188 bp	1.0	81.8
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	CTCCCAGACGTCGTAGAGAA	50.0	55.0	58.0	58.3	1232-1250 bp	1.0	81.5
rps15-ycf1_p3	TCCCAACGCGTTACATAAGG	TTTCTCTCCCAGACGTCGTA	50.0	50.0	58.0	57.8	1255 bp	0.5	58.4
rps15-ycf1_p4	GATGAGTCGAAGAACGCCAT	TTTCTCTCCCAGACGTCGTA	50.0	50.0	58.1	57.8	1130 bp	0.5	57.9
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	TTCTCTCCCAGACGTCGTAG	50.0	55.0	58.0	58.3	1254 bp	0.5	57.8