Hotspot

ycf1-ndhF

SSC · 117041-117112 · 72 bp

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Pi 0.371

MarkerSeek score 75.30

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 72
variable_sites: 23
pi: 0.371
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ACGAAGGGGACTAAACAGGA	TTGGTCATACAATCGCGGTT	50.0	45.0	58.0	57.9	562-616 bp	1.0	91.0
ycf1-ndhF_p2	ACAGGAACAAGAGGGATCCA	TTGGTCATACAATCGCGGTT	50.0	45.0	58.0	57.9	548-602 bp	1.0	90.8
ycf1-ndhF_p3	AAGAGGGATCCACCGAAGAA	TTGGTCATACAATCGCGGTT	50.0	45.0	58.0	57.9	540-594 bp	1.0	90.8
ycf1-ndhF_p4	GCAGAATACCGTCACCCATT	TTGGTCATACAATCGCGGTT	50.0	45.0	58.0	57.9	672-726 bp	1.0	90.2
ycf1-ndhF_p5	ACGAAGGGGACTAAACAGGA	AGCATGACGCAGCCTTTTAT	50.0	45.0	58.0	58.2	999-1053 bp	1.0	87.5