Hotspot

rpl14-rpl16

LSC · 87348-87485 · 138 bp

Back to Ventilago

Pi 0.0511
MarkerSeek score 69.00
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
138
variable_sites
7
pi
0.0511
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 TCAAATGGCTTTGAGGTTGA TGAAATGAGCGGAGTAGCAG 40.0 50.0 55.4 57.7 259-260 bp 1.0 59.7
rpl14-rpl16_p2 TCAAATGGCTTTGAGGTTGA AATAGCGGCATCCAAAATGC 40.0 45.0 55.4 57.5 214-215 bp 1.0 58.5
rpl14-rpl16_p3 TCAAATGGCTTTGAGGTTGAA TGAAATGAGCGGAGTAGCAG 38.1 50.0 56.3 57.7 259-260 bp 1.0 58.1
rpl14-rpl16_p4 ATGGCTTTGAGGTTGAATCA TGAAATGAGCGGAGTAGCAG 40.0 50.0 55.2 57.7 255-256 bp 1.0 57.8
rpl14-rpl16_p5 TGGCTTTGAGGTTGAATCAT TGAAATGAGCGGAGTAGCAG 40.0 50.0 55.2 57.7 254-255 bp 1.0 57.8