Hotspot

trnF-GAA-ndhJ

LSC · 52936-53756 · 821 bp

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Pi 0.0463

MarkerSeek score 85.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 36
pi: 0.0463
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	TGGATAGGATGGCCTTTACG	55.0	50.0	58.0	56.8	960-1000 bp	1.0	76.7
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	GGATAGGATGGCCTTTACGT	55.0	50.0	58.0	56.8	959-999 bp	1.0	76.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	924-964 bp	1.0	76.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	923-963 bp	1.0	76.5
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGATGGCC	55.0	47.6	58.0	57.7	967-1007 bp	1.0	76.0