Hotspot

psaA-ycf3

LSC · 45427-46399 · 973 bp

Back to Ventilago

Pi 0.0277

MarkerSeek score 74.00

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 973
variable_sites: 21
pi: 0.0277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCTGCCGAGTATTGG	50.0	50.0	57.9	58.0	937-1151 bp	1.0	84.4
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	879-1093 bp	1.0	84.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	CGATCAAGCTGCCGAGTATT	50.0	50.0	57.9	58.1	939-1153 bp	1.0	83.5
psaA-ycf3_p4	GAGAAATGACCTGGTCTGGC	ATCAAGCTGCCGAGTATTGG	55.0	50.0	58.3	58.0	955-1169 bp	1.0	83.2
psaA-ycf3_p5	GAGAAATGACCTGGTCTGGC	AGCGCAGAATTGGTTGAAGA	55.0	45.0	58.3	58.1	897-1111 bp	1.0	82.8