Hotspot

trnR-UCU-atpA

LSC · 11731-12444 · 714 bp

Back to Ventilago

Pi 0.0632

MarkerSeek score 70.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 714
variable_sites: 22
pi: 0.0632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.49
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	492-858 bp	1.0	65.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	GGAAGCCCTTTTGAAAGAAGC	42.9	47.6	57.4	58.0	491-857 bp	1.0	65.7
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GGAAGCCCTTTTGAAAGAAGC	47.6	47.6	57.3	58.0	431-797 bp	1.0	65.1
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATTG	TCAGGAACAGATGGAACGTT	42.9	45.0	57.4	56.8	468-834 bp	1.0	64.3
trnR-UCU-atpA_p5	TGGAATGAAAAGCGTCCATTG	TTCAGGAACAGATGGAACGT	42.9	45.0	57.4	56.8	469-835 bp	1.0	64.3