Hotspot

psbK-psbI

LSC · 8282-8729 · 448 bp

Back to Ventilago

Pi 0.0276

MarkerSeek score 64.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 12
pi: 0.0276
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.1	58.3	569-579 bp	1.0	81.1
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	AGGATTACGCCCTGGATCAT	50.0	50.0	57.9	58.3	564-574 bp	1.0	81.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	561-571 bp	1.0	79.1
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	562-572 bp	1.0	79.1
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	AGGATTACGCCCTGGATCAT	45.0	50.0	58.3	58.3	563-573 bp	1.0	79.1