Hotspot

trnK-UUU-rps16

LSC · 4515-5515 · 1001 bp

Back to Ventilago

Pi 0.0302

MarkerSeek score 73.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 30
pi: 0.0302
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCGGTCTTACAAACTC	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1181-1212 bp	1.0	81.8
trnK-UUU-rps16_p2	GTCGCGGTCTTACAAACTCT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	57.9	58.0	1180-1211 bp	1.0	81.8
trnK-UUU-rps16_p3	CCGATGGTATGGACGAATCC	ATTTCAAAGAAAGCGGGGGT	55.0	45.0	57.9	58.0	1159-1190 bp	1.0	81.7
trnK-UUU-rps16_p4	CAGTCGCGGTCTTACAAACT	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	1182-1213 bp	1.0	80.6
trnK-UUU-rps16_p5	TCAGTCGCGGTCTTACAAAC	ATTTCAAAGAAAGCGGGGGT	50.0	45.0	58.2	58.0	1183-1214 bp	1.0	80.5