Hotspot

rbcL

LSC · 56249-57703 · 1455 bp

Back to Veitchia

Pi 0.000916

MarkerSeek score 46.80

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1455
variable_sites: 2
pi: 0.000916
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGAGTAGACCTTGTCGTTGT	TATTCGGTTCGGCTCAATCC	50.0	50.0	57.3	57.8	1603 bp	1.0	72.6
rbcL_p2	CGAGTAGACCTTGTCGTTGT	TGGGCCGAGTTTAATTGCAA	50.0	45.0	57.3	58.4	1564 bp	1.0	71.5
rbcL_p3	CGAGTAGACCTTGTCGTTGT	TTGGGCCGAGTTTAATTGCA	50.0	45.0	57.3	58.4	1565 bp	1.0	71.5
rbcL_p4	CGAGTAGACCTTGTCGTTGT	GGAAAAGATTGGGCCGAGTT	50.0	50.0	57.3	58.5	1573 bp	1.0	70.9
rbcL_p5	TCGAGTAGACCTTGTCGTTG	TATTCGGTTCGGCTCAATCC	50.0	50.0	57.0	57.8	1604 bp	1.0	70.4