Hotspot

atpF

LSC · 12026-13338 · 1313 bp

Back to Veitchia

Pi 0.00102

MarkerSeek score 51.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1313
variable_sites: 2
pi: 0.00102
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	AGGGACAACGAAGTGATACA	45.0	45.0	57.4	56.2	1491-1494 bp	1.0	60.7
atpF_p2	ACTAATTTCGTCGGCTCGAA	AAGGGACAACGAAGTGATACA	45.0	42.9	57.4	57.0	1492-1495 bp	1.0	58.8
atpF_p3	ACTAATTTCGTCGGCTCGAA	AGGGACAACGAAGTGATACAA	45.0	42.9	57.4	57.0	1491-1494 bp	1.0	58.8
atpF_p4	GGCTCGAAGGGTTACCATTA	AGGGACAACGAAGTGATACA	50.0	45.0	57.0	56.2	1479-1482 bp	1.0	57.9
atpF_p5	TCTTTTTCGGAAGCAAAGGGA	AGGGACAACGAAGTGATACA	42.9	45.0	58.1	56.2	1441-1443 bp	1.0	57.3