Hotspot

psaC-ndhE

SSC · 119309-119865 · 557 bp

Back to Veitchia

Pi 0.00122

MarkerSeek score 62.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 557
variable_sites: 1
pi: 0.00122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	671-751 bp	1.0	78.1
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	670-750 bp	1.0	78.1
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	691-771 bp	1.0	78.1
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.1	58.8	669-749 bp	1.0	77.0
psaC-ndhE_p5	CAGGCTCGTACACATTGAGT	TGAAGCAGCTATTGGGCTAG	50.0	50.0	57.6	57.4	630-710 bp	1.0	76.1