Hotspot

accD-psaI

LSC · 59983-61009 · 1027 bp

Back to Veitchia

Pi 0

MarkerSeek score 61.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1030-1140 bp	1.0	83.6
accD-psaI_p2	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1031-1141 bp	1.0	83.6
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1036-1146 bp	1.0	83.0
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	1037-1147 bp	1.0	83.0
accD-psaI_p5	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1036-1146 bp	1.0	82.8