Hotspot

trnM-CAU-atpE

LSC · 53402-53581 · 180 bp

Back to Veitchia

Pi 0

MarkerSeek score 54.90

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	TATTGCTTTCATACGGCGGG	GCTGAGGGTAAGAGACAAGC	50.0	55.0	58.4	58.0	315-319 bp	1.0	77.6
trnM-CAU-atpE_p2	TATTGCTTTCATACGGCGGG	GCGAATCTAGCTCTCAGACG	50.0	55.0	58.4	58.0	288-292 bp	1.0	77.6
trnM-CAU-atpE_p3	TATTGCTTTCATACGGCGGG	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.4	58.0	278-282 bp	1.0	77.6
trnM-CAU-atpE_p4	TATTGCTTTCATACGGCGGG	TAGGACACGAGTAGAGGCTG	50.0	55.0	58.4	58.0	265-269 bp	1.0	77.6
trnM-CAU-atpE_p5	TATTGCTTTCATACGGCGGG	CGAGCTAGGACACGAGTAGA	50.0	55.0	58.4	58.1	270-274 bp	1.0	77.4