Hotspot

trnF-GAA-ndhJ

LSC · 48205-48920 · 716 bp

Back to Veitchia

Pi 0.00279

MarkerSeek score 48.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 3
pi: 0.00279
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	826-827 bp	1.0	78.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	816-817 bp	1.0	78.3
trnF-GAA-ndhJ_p3	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.8	57.9	872-873 bp	1.0	77.9
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	869-870 bp	1.0	77.6
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	868-869 bp	1.0	77.6