Hotspot

psaA-ycf3

LSC · 42027-42669 · 643 bp

Back to Veitchia

Pi 0.00104

MarkerSeek score 46.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 643
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	755 bp	1.0	78.0
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	759 bp	1.0	77.8
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATCACGAGGCGTTTCGAATT	50.0	45.0	57.9	58.3	749 bp	1.0	77.1
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	754 bp	1.0	77.0
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	800 bp	1.0	76.5