Hotspot

psbM-trnD-GUC

LSC · 30033-31046 · 1014 bp

Back to Veitchia

Pi 0.00131

MarkerSeek score 47.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1127 bp	1.0	61.6
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TATGCCATACACCTTGCTGG	45.0	50.0	55.8	57.7	1153 bp	1.0	59.5
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CCATACACCTTGCTGGGATT	45.0	50.0	55.8	57.6	1149 bp	1.0	58.8
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.4	57.9	1134 bp	1.0	58.5
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	1144 bp	1.0	58.3