Hotspot

trnS-GCU-trnG-GCC

LSC · 8253-9314 · 1062 bp

Back to Veitchia

Pi 0.000669

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000669
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	1059-1136 bp	1.0	55.5
trnS-GCU-trnG-GCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	1062-1139 bp	1.0	54.4
trnS-GCU-trnG-GCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	1061-1138 bp	1.0	54.4
trnS-GCU-trnG-GCC_p4	AGAAGAAGCGGAGAGAGAGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.2	55.5	1115-1192 bp	1.0	53.9
trnS-GCU-trnG-GCC_p5	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	1060-1137 bp	1.0	53.6