Hotspot

matK-trnK-UUU

LSC · 3356-4074 · 719 bp

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Pi 0.000927
MarkerSeek score 47.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
719
variable_sites
1
pi
0.000927
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CCACGATCATGAGCAAATGTG AACGGTAGAGTACTCGGCTT 47.6 50.0 57.9 58.2 849-850 bp 1.0 70.4
matK-trnK-UUU_p2 CCACGATCATGAGCAAATGTG ACGGTAGAGTACTCGGCTTT 47.6 50.0 57.9 58.2 848-849 bp 1.0 70.4
matK-trnK-UUU_p3 CCACGATCATGAGCAAATGTG GTCGTGCAGTGCAATTCAAT 47.6 45.0 57.9 57.7 929-930 bp 1.0 69.0
matK-trnK-UUU_p4 ACCACGATCATGAGCAAATGT AACGGTAGAGTACTCGGCTT 42.9 50.0 58.3 58.2 850-851 bp 1.0 68.6
matK-trnK-UUU_p5 ACCACGATCATGAGCAAATGT ACGGTAGAGTACTCGGCTTT 42.9 50.0 58.3 58.2 849-850 bp 1.0 68.6