Hotspot

ndhF-rpl32

SSC · 116339-117445 · 1107 bp

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Pi 0.00424

MarkerSeek score 56.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1107
variable_sites: 9
pi: 0.00424
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	40.0	45.0	57.0	57.8	189-1303 bp	1.0	74.6
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAGCTTTCAATGCTGCCCTA	40.0	45.0	57.0	57.8	187-1301 bp	1.0	74.3
ndhF-rpl32_p3	TTGTTGCCGTTGGAAAAAGT	GCTGCCCTATATCCCTTCCT	40.0	55.0	57.0	58.3	188-1290 bp	1.0	73.4
ndhF-rpl32_p4	TTGTTGCCGTTGGAAAAAGT	AGCTTTCAATGCTGCCCTAT	40.0	45.0	57.0	57.6	188-1300 bp	1.0	72.4
ndhF-rpl32_p5	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	38.1	45.0	57.7	57.8	188-1304 bp	1.0	72.4