Hotspot

rpl33-rps18

LSC · 71286-71427 · 142 bp

Back to Vauquelinia

Pi 0.0355

MarkerSeek score 71.60

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 142
variable_sites: 9
pi: 0.0355
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGACACAATACGCCTAGTCG	TCAATTCGATCCCCCGATTG	55.0	50.0	58.5	57.7	188-326 bp	1.0	80.3
rpl33-rps18_p2	TGTCCCTGTTGTTACAAGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.3	57.7	188-287 bp	1.0	79.1
rpl33-rps18_p3	GCATACGATTCATGGGGAGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	187-270 bp	1.0	78.3
rpl33-rps18_p4	GACACAATACGCCTAGTCGA	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	187-325 bp	1.0	77.8
rpl33-rps18_p5	TCGACACAATACGCCTAGTC	TCAATTCGATCCCCCGATTG	50.0	50.0	57.2	57.7	188-327 bp	1.0	77.8