Hotspot

trnP-UGG-psaJ

LSC · 70051-70467 · 417 bp

Back to Vauquelinia

Pi 0.0044

MarkerSeek score 38.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 3
pi: 0.0044
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	TACCAAACTGCGCTACATCC	AGTACTTACTACCGGTGCCA	50.0	50.0	58.0	57.8	80-487 bp	1.0	78.7
trnP-UGG-psaJ_p2	TACCAAACTGCGCTACATCC	GTACTTACTACCGGTGCCAC	50.0	55.0	58.0	57.7	188-486 bp	1.0	78.2
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	AGTACTTACTACCGGTGCCA	50.0	50.0	58.1	57.8	187-520 bp	1.0	78.0
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	AGTACTTACTACCGGTGCCA	50.0	50.0	58.1	57.8	187-519 bp	1.0	78.0
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	AGTACTTACTACCGGTGCCA	50.0	50.0	58.1	57.8	187-531 bp	1.0	77.9