Hotspot

trnG-GCC-trnfM-CAU

LSC · 39341-39527 · 187 bp

Back to Vauquelinia

Pi 0.00269

MarkerSeek score 59.60

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 1
pi: 0.00269
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	80-265 bp	1.0	82.7
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	AGTTCTTCTATGCATCGCGG	50.0	50.0	57.8	58.1	80-320 bp	1.0	81.8
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	188-263 bp	1.0	81.5
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	188-286 bp	1.0	81.2
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	188-285 bp	1.0	81.2